Cits857
WebApr 17, 2013 · Primers were designed to amplify the entire sequences of pBV-DsRed and pBV-OPH, except the cIts857 gene which encodes the repressor protein for transcriptional control in plasmid pBV220 (primers used were no. 5 and no. 6, see Table 1). The amplified fragment was then self-circularized to construct the plasmids pL-DsRed and pL-OPH. WebThe galR gene, which encodes the Gal repressor protein in Escherichia coli, has been fused to the strong pL promoter of bacteriophage lambda in plasmid pKC31. The pL promoter is kept repressed by a thermolabilie lambda repressor, CIts857, to prevent cell killing. Heat induction of the pL-galR fusion plasmid synthesizes large amounts of active Gal …
Cits857
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WebThe prophage lambda cIts857 induction test with Escherichia coli K12 envA uvrB as the lysogen has been successfully applied to the screening of sparingly water-soluble carcinogens that have been dissolved in dimethyl sulfoxide and metabolically activated with liver enzymes induced either with Aroclor 1254 or phenobarbital. Growth of noninduced ... WebXX RN [3] RC pJLf201 from phage fd terminator & lambda, cIts857 gene/pR/pL RA McCarthy J.E., Schairer H.U., Sebald W.; RT "Translational initiation frequency of atp genes from Escherichia RT coli: identification of an intercistronic sequence that enhances RT translation"; RL EMBO J. 4:519-526(1985).
WebFeb 15, 1987 · The galR gene, which encodes the Gal repressor protein in Escherichia coli, has been fused to the strong pL promoter of bacteriophage lambda in plasmid pKC31. The pL promoter is kept repressed by a thermolabilie lambda repressor, CIts857, to … WebThe prophage lambda cIts857 induction test with Escherichia coli K12 envA uvrB as the lysogen has been successfully applied to the screening of sparingly water-soluble …
WebThis was isolated as spontaneous mutant of a lambda Nam7 Nam53 cIts857 prophage. Mycoplasma contamination Not detected Technical information ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. WebThe vector pBV220 contained the λ promoters P R and P L in tandem orientation, the cIts857 gene coding for the thermolabile repressor, and two transcription terminators.
WebSep 1, 1990 · In OOP RNA-cll mRNA structures containing a potential region of continuous double-stranded RNA bounded by a non-complementary unpaired region, RNase III cleaved the ell mRNA at one or more...
WebMar 30, 2024 · 4h 27m. Friday. 03-Mar-2024. 07:21PM AEDT Canberra Int'l - CBR. 08:36PM AWST Perth Int'l - PER. B738. 4h 15m. Join FlightAware View more flight … smart baby giftsWebSep 8, 2024 · 在高温 (45℃)时，cIts857蛋白失活，阻遏解除，促使PL启动子转录。 系统由于受cIts857作用，尤其适合于表达对大肠杆菌有毒的基因产物，缺点是温度转换不仅可 … hill farm glamping warwickshirehttp://genome-www.stanford.edu/vectordb/vector_descrip/NOTCOMPL/PJW2.SEQ.html hill farm caravan park romseyWebJan 1, 1999 · A high level expression vector pRC has been constructed, which contains P_ (R) promoter, cIts857 gene, multiple cloning sites (MCS) and two strong transcription terminators. With this vector, hTNF-alpha, hIL-3 and anti-HEL McAb Fd genes have been expressed successfully, and their products each account for more than 36% of the total … smart baby feeding bottleWebNov 30, 1990 · Experiments performed over a temperature range spanning 29-42 degrees C indicate that, under our conditions, temperatures as low as 29 degrees C may be … smart baby girl nameshill farm gosbeckWebCIts857 expression suppressor gene, regulated by temperature, PRPL tandem promoter, rrnB or rrnbT1T2 terminator, Amp Ampicillin, Ori origin Source publication +1 Hydroxylation of Human Type III... hill farm great wigborough