Cst myc antibody
WebThe FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions. The FLAG tag may be inserted at the N terminus, the N terminus preceded by a methionine residue, the C terminus, or internal positions of the target protein. WebHuman c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max. It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains.
Cst myc antibody
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Webc-Myb Antibody (D-7) is a mouse monoclonal IgG 2a κ c-Myb antibody, cited in 19 publications, provided at 200 µg/ml. c-Myb Antibody (D-7) is recommended for detection … WebCells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue). See ...
WebApr 13, 2024 · DNA fragments were incubated overnight with specific antibody c-Myc (E5Q6W, CST, USA) and control IgG. The next day, antibody-binding magnetic beads were added. Proteinase K was used to elute and digest the immunoprecipitated product. The purified DNA product was used for subsequent PCR detection (Table S2). Mouse IgG … Webc-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth ...
Web13 hours ago · Our previous studies demonstrated that rapamycin and rapalogs significantly induced IRES-mediated translation of cyclin D1 and c-myc mRNAs following exposure [16, 20, 21].The ability of these compounds to induce IRES activity of these two determinants was sufficient to render cells resistant to rapamycin in an AKT-dependent manner … WebGeneral description. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper. [ 1]
WebThe mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene.
WebWe hypothesized that Euonymus sachalinensis (ES) induces apoptosis by inhibiting the expression of c-Myc in colon cancer cells, and this study proved that the methanol extract of ES has anticancer effects in colon cancer cells. ES belongs to the Celastraceae family and is well known for its medicinal properties. Extracts of species belonging to this family … diamond shaped beauty marksWebACTL6B/BAF53B is a member of the neural-specific SWI/SNF complex that facilitates binding to target genes and is involved in memory and synaptic plasticity (13-15). ACTL6/BAF53 has been shown to interact with c-Myc, where it functions as a cofactor and is important in the transformation process (16). Further studies have shown … diamond shaped boardWebDec 4, 2015 · The antibody is question is the cell signaling Myc-tag antibody and I am hoping to use mouse tissues in an upcoming ChIP experiment. ... It might be more important to have a very nice anti-MYC ... cisco power stack cable diagramWeb1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human c-MYC (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at dilution 1:1000 (green), and 0.2 ug … diamond shaped bookcaseWebThis antibody detects c-myc protein and c-myc tagged proteins. MA1-980 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA, flow cytometry, and … cisco predictive networkWebICC staining of purified anti-c-Myc antibody (clone 9E10) on HeLa cells transfected with multi-tag plasmid containing c-Myc epitope sequence. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100, and blocked with 5% fetal bovine serum in PBS for 1 hour. The cells were then incubated with 1 µg/mL of the ... cisco power stack cablesWebMyc Antibody (9E10) is an IgG1 κ mouse monoclonal Myc antibody (also designated c-Myc antibody) that detects the Myc protein of mouse, rat, human, monkey, feline and canine origin by WB, IP, IF, IHC(P), FCM … diamond shaped bookcase moonlight