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WebpX458-Ef1a-Cas9-H2B-mCherry (Plasmid #159655) Print Enlarge View all sequences Purpose (Empty Backbone) Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter. Depositing Lab Joseph Brzezinski Publication WebApr 1, 2015 · Instead of mKO2 and mAG, the FUCCI 2 mice use mCherry-hCdt1 30–120 and mVenus-hGem 1–110 (Figure 3 (b)). These sensors produce better color contrast and can be spectrally separated from GFP. 16 Another problem of the first generation FUCCI mice is that the probes are expressed at very low levels in certain tissues. WebFeb 16, 2024 · To analyse the subcellular localization of PhOBF1, the fusion protein PhOBF1-GFP was transiently expressed in tobacco leaves with a nucleus-localized marker H2B-mCherry. Green fluorescent signals of PhOBF1-GFP were observed to overlap with red fluorescent signals of H2B-mCherry , suggesting that PhOBF1 was located in the … launceston to hobart drive