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Phellsgate 2

WebMar 20, 2009 · The amplicons were cloned in pHELLSGATE 2 using BP and LR clonase. This protocol allows the insertion, in one step, of the genes of interest in sense and antisense orientation with an intron in... WebAfter inoculation with F. solani for 72 h, the PnPR-like expression level in the control leaves was about 5-times higher than that in the leaves transformed with pHellsgate 2-PnPR-like, which ...

A high-throughput inducible RNAi vector for plants - PubMed

WebSep 1, 2001 · Cloning vector pHELLSGATE spectinomycin resistance protein(ENA - CAC86253) Cloning vector pHELLSGATE (ENA - AJ311874) Cloning vector pKANNIBAL (ENA - AJ311873) Go to all (7) records in ENA Reviews Reviews, recommendations & commentary from expert sources. Faculty Opinions (2) … WebBioz Stars score, Techniques, Protocol Conditions and more for Binary Vector Phellsgate 2, supplied by Thermo Fisher. Data for Binary Vector Phellsgate 2 gathered from related … dr yao appleton wi https://deardiarystationery.com

Suppression of GhAGP4 gene expression repressed the

http://www.biofeng.com/zaiti/zhiwu/pHELLSGATE.html WebThe pHELLSGATE vectors contain two recombination cassettes consisting of either attP1-ccdB-attP2 or attR1-ccdB-attR2 in an inverted repeat configuration, such that when gene … WebAug 1, 2003 · The pHELLSGATE vectors contain convenient restriction sites that can be used to check for the presence of inserted sequences and diagnose the orientation of the … comic book strip template

Bidirectional cross-kingdom RNAi and fungal uptake of external …

Category:Phytochrome RNAi enhances major fibre quality and agronomic

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Phellsgate 2

pHELLSGATE 12 Sequence and Map - SnapGene

WebApr 5, 2024 · [0072] 实施例4：mswrky41基因转化紫花苜蓿的获得 [0073] 农杆菌准备：将在-80℃冰箱中保存的phb-mswrky41-flag和phellsgate-mswrky41rnai农杆菌菌株分别在含有抗生素为kan50+rif100和spe50+rif100的yeb固体培养基上划线活化，28℃培养48h后，挑取单克隆至含有相同抗生素的yeb液体培养 ...

Phellsgate 2

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WebpHELLSGATE is an efficient, high-throughput and effective plant gene silencing vector. Vector Type. Gene Silencing Vector, Gateway Vector, Plant Dual Expression Vector ... Powder; Solution Size. 2 μg; 10 μL . High Copy/Low Copy. High Copy . Prokaryotic Resistance. Spectinomycin . Length. 18691 bp . Selectable Marker. WebIn this paper we describe two improved pHellsgate vectors that facilitate rapid generation of hpRNA-encoding constructs. pHellsgate 4 allows the production of an hpRNA construct in …

WebSep 19, 2016 · The plasmids pHellsgate-Bc-DCL1/2 and pHellsgate-Bc+Vd-DCLs were constructed following the methods outlined for the Hellsgate8.0 system 51. The Bc-DCL1/2 RNAi fragment was obtained by integrating Bc-DCL1 (252 bp) and Bc-DCL2 (238 bp) via overlapping PCR. Web豆丁网是面向全球的中文社会化阅读分享平台，拥有商业,教育,研究报告,行业资料,学术论文,认证考试,星座,心理学等数亿实用 ...

WebJan 13, 2024 · The pHellsgate 2-PnPR-like recombinant plasmid and an empty pHellsgate two vector were inserted into separate A. tumefaciens EHA105 cells. Positive clones were … WebDec 23, 2001 · The pHELLSGATE vector was tested using 200 and 400 nt PCR products for two different genes. Many bacterial colonies were obtained on chloramphenicol …

WebDOI: 10.1111/j.1467-7652.2005.00149.x Abstract We describe here a vector system that allows dexamethasone-inducible RNAi against plant genes. The system utilizes a modified pHELLSGATE vector, under the control of the pOp6 promoter, and …

WebpHELLSGATE, pWATERGATE, pSTARGATE and pOpOff contain Gateway TM recombination sites. Gateway TM is proprietary technology owned by Invitrogen and to the extent that CSIRO vectors incorporate Gateway TM technology, ... 5.2 ALL TERMS, CONDITIONS AND WARRANTIES (INCLUDING ANY WARRANTY AS TO MERCHANTABLE QUALITY OR … dr yao chang fort worthWebpHELLSGATE载体价格10000元，pHELLSGATE载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性壮观霉素或链霉素，质粒大小为18691BP，测序引物见 … comic book stuffWebThe system utilizes a modified pHELLSGATE vector, under the control of the pOp6 promoter, and the synthetic transcription factor, LhGR. We demonstrate that the production of RNAi … comic books trading cards