Phospho specific antibody
WebApr 9, 2024 · “A Pan-specific Antibody for Direct Detection of Protein Histidine Phosphorylation” The idea is that the antibody is: ‘pan’ in that it will react with all phospho-histidine residues on proteins, but ‘specific’ in that it will not react with unphosphorylated histidine residues or other phospho-aminoacids e.g. phospho-serine. Share WebFeatured Phospho-specific Antibodies: AKT/PDPK1. Akt, also named Protein Kinase B (PKB), is a serine/threonine-specific protein kinase, including the most important two, Akt1 and Akt2. The former one can inhibit apoptotic processes in cellular survival pathways and induce skeletal muscle hypertrophy and general tissue growth in cellular ...
Phospho specific antibody
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WebPhospho-Specific Antibodies For analyzing cell signaling pathway activation Our phospho-specific antibody range References For Analyzing Cell Signaling Pathway Activation View … WebThe total and phospho-specific antibody can be purchased individually. Protein Phosphorylation in Disease Research Phosphorylation is involved in the regulation of multiple biological processes and appropriate phosphorylation is crucial for cell homeostasis. Kinases are enzymes that catalyze the transfer of a phosphate group to a …
WebPhospho-specific antibodies are used not only to detect phosphorylated proteins or peptides, but also to measure changes in their level and specificity of modification by processes of phosphorylation. BiologicsCorp can provide a wide variety of phospho-specific antibodies with our professional techniques. WebIn general, these involve the use of phospho-specific antibodies and include microplate-based (View Proteome Profiler 96 Antibody Arrays) and membrane-based (View Proteome Profiler Antibody Arrays) detection formats (Box 1, Figures 7 and 8). 17, 18 The obvious benefit of these assays is that throughput capability is greatly enhanced by ...
WebJun 5, 2015 · The eight phospho-specific antibodies were chosen to represent major signaling nodes responsive to cytokine, TLR, and antigen receptor signaling. This antibody panel is used with 8 standard stimulation conditions (unstimulated, IFNa, IL-6, IL-7, IL-10, IL-21, LPS, PMA+ ionomycin), although other stimuli can be added. WebAsc (Tyr-144), phospho-specific Antibody Catalog # AP5631 Asc (Tyr-144), phospho-specific Antibody Rabbit Polyclonal Application / Dilution Size 100 μl Species Reactivity Hu, Rt, Ms MW 22 kDa $309 Add to Cart DATASHEET Background Images Sequence Specificity Buffer & Storage References Reviews
WebPhospho-specific antibodies Antibodies that discriminate between the phosphorylated and the non-phosphorylated state of a protein, is one of the most frequently asked for types of PTM specific antibodies. These could be made as monoclonal antibodies but are more often produced as antigen specific affinity purified polyclonal antibodies.
WebPhospho-specific Antibodies. Creative Diagnostics offers extensive selection of phosphor-specific antibodies, which can be used in a variety of applications including Western blot, … include game.hWebab138668 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. ... (phospho T182) antibody (ab138668) at 1/500 dilution + K562 cell lysate (from cells ... include gcses on cvWebThe NeuroMab™ anti-Phospho-p53 (Ser15) antibody (Rodent Specific) recognizes endogenous levels of p53 protein only when phosphorylated at Ser15. Expression Host Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser15 of mouse p53 protein. inc r1里的寻址方式是http://www.innovagen.com/phospho-specific-antibodies include generateexportheaderWebA more direct approach for generating site-specific phospho antibodies utilizes phosphorylated forms of synthetic peptides. Short peptides made of the 15-20 amino acids immediately surrounding the phosphorylated amino acid of interest focus the immune response to just that specific phosphosite. Moreover, these short peptides are generally ... include garage in square footageWebBuffers and proteins which contain phosphate should be avoided with this antibody. Certain proteins known to contain phosphorylated serine and threonine may not be detected by … include functionalWebPhospho-specific antibodies can be absorbed onto a nonphosphorylated peptide column to remove nonspecific antibody molecules. Supplement the antibody solution with 10 µg/mL … include getch.h