Webb11 mars 2024 · Our scRNA-seq, sc-qPCR and FISH analyses of Pmch-expressing neurons largely substantiates this dichotomy and sheds new light on the molecular … WebbscRNA-Seq can be used to screen mutant phenotype in the hypothalamus. (a) tSNE showing distribution of diencephalic and nearby brain structures in control (Foxd1 Cre/+ ) and mice selectively ...
Single cell RNA-Seq analysis identifies molecular mechanisms ...
Webb1 nov. 2024 · Representative marker genes for MERFISH analysis were selected after scRNA-seq of the preoptic area, ... has been explored in mouse hypothalamus in order to trace cell lineages. Webb24 okt. 2024 · We performed a comprehensive survey of DR and MR serotonin neurons in the adult mouse brain by scRNA-seq ().To specifically label serotonin neurons, we crossed Sert-Cre mice (Gong et al., 2007) with the tdTomato Cre reporter mouse, Ai14 (Madisen et al., 2010). (Serotonin transporter, or Sert, encoded by the gene Slc6a4, is a marker for … hyundai grand i10 cc
(PDF) Expansion-Assisted Iterative-FISH defines lateral hypothalamus …
WebbWe used mice between 10 and 16 wk of age for scRNA-seq and mice between 8 and 25 wk of age for immunohistochemistry. As previously described ( 6 ), Aire −/− mice develop a chronic-progressive spontaneous uveoretinitis, as seen in the fundus images and fluorescein angiograms obtained from one Aire −/− mouse at 5 time points over 20 wk ( … Webb18 dec. 2024 · To investigate the potential role of RIIβ‐PKA in the DMH GABAergic neurons for the regulation of WAT browning, a series of genetically modified mouse strains, single‐cell sequencing (scRNA‐seq), transcriptome sequencing (RNA‐seq), whole‐cell patch‐clamp technique, designer receptors exclusively activated by designer drugs … Webbför 14 timmar sedan · The neural stem cells (NSCs) in the hypothalamus are relatively more narrowly defined than in other neurogenic regions of the postnatal brain. By leveraging single-cell RNA sequencing (scRNA-seq) data, we generated an integrated reference dataset comprising 296,282 cells from postnatal hypothalamic regions and … molly grantham nc