Tth ssb
WebDescription. Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does … WebThis “step up” feature of the SSB reflects the objective of the Savings Bond programme, which is to facilitate long-term savings and investment. Coupon rates for each issuance … Please note that you will not be able to amend or cancel submitted redemption …
Tth ssb
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WebJun 21, 2006 · To study the biochemical properties of single-stranded DNA-binding (SSB) protein from Deinococcus geothermalis (DgeSSB), we have cloned the ssb gene obtained by PCR and developed an overexpression system. The gene consists of an open reading frame of 900 nucleotides encoding a protein of 300 amino acids with a calculated molecular … WebJan 1, 2006 · The Tth SSB-255 mutant protein was also purified using the same strategy (data not shown). The final products were shown to be 99% pure. Properties of Tth SSB …
WebExcellgen Tth-SSB [EG-171] - Return to List . Next WebApr 5, 2008 · The thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. coli …
WebDrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical purposes. We report the identification and characterization of the single-stranded DNA-binding protein (SSB) from the mesophile and highly radiation-resistant Deinococcus … WebIncluding ET SSB in TtAgo reactions can improve cleavage of higher GC content substrates In some cases the addition of ET SSB ( NEB #M2401 ) can aid in the cleavage of higher GC content dsDNA substrates.1 ET SSB must be titrated to find an optimal concentration for the application, and is only found to be beneficial when guides are fully complementary to the …
WebTtAgo is a prokaryotic argonaute from the Gram-negative, thermophilic bacterium Thermus thermophilus which functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5′-phosphorylated single-stranded DNA oligonucleotide guide. The RNase H-like active site of TtAgo requires divalent Mg 2+ (or Mn 2+) (See product usage note #1) …
WebMay 17, 2006 · The wild-type T.thermophilus SSB and its mutant were prepared as described in Materials and Methods, which were named TthSSB and TthSSB-255 protein, respectively. Each step of the purification of Tth SSB was analyzed by 12.5% SDS–PAGE with CBB staining ( Figure 1 ). iprompt medical groupWebTth SSB and Taq SSB have a much lower content of Gly residues than Eco SSB (Table 1). Most of the Gly residues of the thermostable SSB proteins are homologous to conserved Gly in the Eco SSB ... ipromx twitterWebApr 6, 2024 · On April 11, 2024 at 15:47:36 ET an unusually large $175.00K block of Call contracts in South State (SSB) was sold, with a strike price of $70.00 / share, expiring in … iprompt telephone numberWebSSB is known to bind ssDNA, protecting it from cleavage by endonucleases. 4 Furthermore, SSB has been found to reduce secondary structure of ssDNA, improve PCR specificity, … ipromx rediffWebOct 1, 2002 · The expression was induced by 0.5 mM IPTG, but it was accompanied by strong inhibition of culture growth.The best induction of thermostable SSB-like proteins … ipromx gtaWebAfter binding single-stranded DNA, SSB destabilizes helical duplexes, thereby allowing DNA polymerases to access their substrate more easily. SSB is involved in DNA replication and … orc r.c. 2305.113WebAug 7, 2014 · We therefore investigated whether a SSB protein could modify the slippage properties of Pab pols. Thermus thermophilus (Tth) SSB stimulates DNA synthesis of Tth DNA polymerase and the heterologous DNA polymerase from the Archaea P. furiosus (Perales et al., 2003). ipromx ytb